Downregulation of CLDN6 inhibits cell migration and invasion and promotes apoptosis by regulation of the JAK2/STAT3 signaling pathway in hepatocellular carcinoma

ABSTRACT:

Background

High expression of CLDN6 in hepatocellular carcinoma (HCC) has been widely reported. During this research, CLDN6’s effect on the infiltration, migration, and apoptosis of HCC cells was investigated.

Methods

Initially, the knockdown and overexpression of CLDN6 in HCC cells were carried out by short interfering RNA (siRNA) and plasmid transfection. The transfection efficiency was detected by means of a quantitative real-time polymerase chain reaction (qRT-PCR) assay, immunofluorescence staining, and Western blot analysis. Transwell and wound-healing assays were employed for the detection of invasion and migration ability. CCK-8 assay and flow cytometry were utilized for the detection of apoptosis. Finally, analysis of the expression of pathway-related proteins (J

SUBMITTER: He Z 

PROVIDER: S-EPMC10425638 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature