Dataset Information

Downregulation of CLDN6 inhibits cell migration and invasion and promotes apoptosis by regulation of the JAK2/STAT3 signaling pathway in hepatocellular carcinoma.

ABSTRACT:

Background

High expression of CLDN6 in hepatocellular carcinoma (HCC) has been widely reported. During this research, CLDN6's effect on the infiltration, migration, and apoptosis of HCC cells was investigated.

Methods

Initially, the knockdown and overexpression of CLDN6 in HCC cells were carried out by short interfering RNA (siRNA) and plasmid transfection. The transfection efficiency was detected by means of a quantitative real-time polymerase chain reaction (qRT-PCR) assay, immunofluorescence staining, and Western blot analysis. Transwell and wound-healing assays were employed for the detection of invasion and migration ability. CCK-8 assay and flow cytometry were utilized for the detection of apoptosis. Finally, analysis of the expression of pathway-related proteins (JAK2, STAT3, p-JAK2, and p-STAT3) and the regulation of apoptotic responses (by measurement of cleaved caspase-3, Bax, and Bcl-2 levels) was carried out.

Results

When CLDN6 was knocked down, the cellular invasion and migration ability decreased, and apoptosis increased, which decreased p-JAK2, p-STAT3, and anti-apoptotic protein bcl-2 expression. Furthermore, an elevation was observed in cleaved caspase-3 and Bax expression levels. Contrarily, upon overexpression of CDLN6, the aforementioned experimental results were reversed.

Conclusions

CLDN6 knockdown results in the inhibition of HCC cells' infiltration and migration and promotes apoptosis via downregulation of the JAK2/STAT3 signaling pathway.

SUBMITTER: He Z

PROVIDER: S-EPMC10425638 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature

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Publications

Downregulation of <i>CLDN6</i> inhibits cell migration and invasion and promotes apoptosis by regulation of the JAK2/STAT3 signaling pathway in hepatocellular carcinoma.

He Zhiyi Z Fan Fangtian F Xu Zhidong Z Zhang Mei M Zhao Rui R Ke Xiquan X Wang Qizhi Q Yan Shanjun S Zheng Hailun H

Translational cancer research 20230710 7

<h4>Background</h4>High expression of <i>CLDN6</i> in hepatocellular carcinoma (HCC) has been widely reported. During this research, <i>CLDN6</i>'s effect on the infiltration, migration, and apoptosis of HCC cells was investigated.<h4>Methods</h4>Initially, the knockdown and overexpression of <i>CLDN6</i> in HCC cells were carried out by short interfering RNA (siRNA) and plasmid transfection. The transfection efficiency was detected by means of a quantitative real-time polymerase chain reaction ...[more]

PMID: 37588735

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