PORCN promotes hepatocellular carcinogenesis via Wnt/β-catenin-dependent manner

ABSTRACT:

Background

Porcupine O-acyltransferase (PORCN), a membrane-bound O-acyltransferase, is crucial in Wnt ligand palmitoylation. However, the roles of PORCN in the development of hepatocellular carcinoma (HCC) remain unknown.

Methods

Western blot, real-time quantitative polymerase chain reaction (RT-qPCR) assays, and The Cancer Genome Atlas (TCGA) database were used to study the expression and prognostic values of PORCN in patients with HCC. Following this, Cell Counting Kit-8 (CCK-8), wound-healing tests, Transwell assay, and a xenograft mouse model were employed to examine the effect of PORCN on HCC cells. Finally, the underlying molecular mechanisms involved in cell proliferation and migration caused by PORCN were identified.

Results

The protein and messenger RNA

SUBMITTER:  

PROVIDER: S-EPMC10425655 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature