Cohesin-mediated DNA loop extrusion resolves sister chromatids in G2 phase.

ABSTRACT: Genetic information is stored in linear DNA molecules, which are highly folded inside cells. DNA replication along the folded template path yields two sister chromatids that initially occupy the same nuclear region in an intertwined arrangement. Dividing cells must disentangle and condense the sister chromatids into separate bodies such that a microtubule-based spindle can move them to opposite poles. While the spindle-mediated transport of sister chromatids has been studied in detail, the chromosome-intrinsic mechanics presegregating sister chromatids have remained elusive. Here, we show that human sister chromatids resolve extensively already during interphase, in a process dependent on the loop-extruding activity of cohesin, but not that of condensins. Increasing cohesin's looping capab

SUBMITTER: Batty P 

PROVIDER: S-EPMC10425840 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature