A high-salt diet promotes hypertrophic scarring through TRPC3-mediated mitochondrial Ca²⁺ homeostasis dysfunction.

ABSTRACT: Diet High in salt content have been associated with cardiovascular disease and chronic inflammation. We recently demonstrated that transient receptor potential canonical 3 (TRPC3) channels regulate myofibroblast transdifferentiation in hypertrophic scars. Here, we examined how high salt activation of TRPC3 participates in hypertrophic scarring during wound healing. In vitro, we confirmed that high salt increased the TRPC3 protein expression and the marker of myofibroblast alpha smooth muscle actin (α-SMA) in wild-type mice (WT) primary cultured dermal fibroblasts but not Trpc3^-/- mice. Activation of TRPC3 by high salt elevated cytosolic Ca²⁺ influx and mitochondrial Ca²⁺ uptake in dermal fibroblasts in a TRPC3-dependent manner. High salt activ

SUBMITTER: Xia W 

PROVIDER: S-EPMC10425910 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature