Genotype diversity of brucellosis agents isolated from humans and animals in Greece based on whole-genome sequencing.

ABSTRACT:

Background

Brucellosis is a zoonotic disease whose causative agent, Brucella spp., is endemic in many countries of the Mediterranean basin, including Greece. Although the occurrence of brucellosis must be reported to the authorities, it is believed that the disease is under-reported in Greece, and knowledge about the genomic diversity of brucellae is lacking.

Methods

Thus, 44 Brucella isolates, primarily B. melitensis, collected between 1999 and 2009 from humans and small ruminants in Greece were subjected to whole genome sequencing using short-read technology. The raw reads and assembled genomes were used for in silico genotyping based on single nucleotide substitutions and alleles. Further, specific genomic regions encoding putative virulence genes were screened for charac

SUBMITTER: Brangsch H 

PROVIDER: S-EPMC10426126 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature