FAAP100 is required for the resolution of transcription-replication conflicts in primordial germ cells.

ABSTRACT:

Background

The maintenance of genome stability in primordial germ cells (PGCs) is crucial for the faithful transmission of genetic information and the establishment of reproductive reserve. Numerous studies in recent decades have linked the Fanconi anemia (FA) pathway with fertility, particularly PGC development. However, the role of FAAP100, an essential component of the FA core complex, in germ cell development is unexplored.

Results

We find that FAAP100 plays an essential role in R-loop resolution and replication fork protection to counteract transcription-replication conflicts (TRCs) during mouse PGC proliferation. FAAP100 deletion leads to FA pathway inactivation, increases TRCs as well as cotranscriptional R-loops, and contributes to the collapse of replication forks a

SUBMITTER: Xu W 

PROVIDER: S-EPMC10426154 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature