Klhl14 Antisense RNA is a Target of Key Skeletogenic Transcription Factors in the Developing Intervertebral Disc.

ABSTRACT:

Study design

RNA in situ hybridization (RISH) allows for validation and characterization of the long noncoding (lnc) natural antisense RNA (NAT) Klhl14as in the embryonic murine intervertebral disc (IVD) in the context of loss-of-function mutants for key transcription factors (TFs) in axial skeleton development.

Objective

Validation of Klhl14as in the developing murine IVD.

Summary of background data

The IVD is a focus of regenerative medicine; however, processes and signaling cascades resulting in the different cell types in a mature IVD still require clarification in most animals including humans. Technological advances increasingly point to implications of lnc NATs in transcription/translation regulation. Transcriptome data generation and analysis identified a prot

SUBMITTER: Kraus P 

PROVIDER: S-EPMC10426336 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature