Application of quantitative real-time PCR to detect Mink Circovirus in minks, foxes and raccoon dogs in northern China.

ABSTRACT: Mink circovirus disease caused by Mink Circovirus (MiCV) is a serious infectious disease of mink that has become prevalent in recent years in China, severely affecting the reproductive performance of mink and causing significant economic losses to farms. To date, there have been few studies on MiCV, its pathogenic mechanism is not clear, and there is no effective vaccine or drug to prevent and control the disease. Therefore, it is necessary to establish a rapid and reliable molecular diagnostic method, which would aid future studies of this novel virus. In our study, we developed a sensitive and specific TaqMan-based quantitative real-time PCR assay targeting the MiCV Cap gene. The assay showed no cross-reaction with other tested animal viruses. The assay is highly sensitive, with a

SUBMITTER: Liu Y 

PROVIDER: S-EPMC10426742 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature