A Spectrofluorometric Method for Real-Time Graft Assessment and Patient Monitoring.

ABSTRACT: Biomarkers are powerful clinical diagnostics and predictors of patient outcome. However, robust measurements often require time and expensive laboratory equipment, which is insufficient to track rapid changes and limits direct use in the operating room. Here, this study presents a portable spectrophotometric device for continuous real-time measurements of fluorescent and non-fluorescent biomarkers at the point of care. This study measures the mitochondrial damage biomarker flavin mononucleotide (FMN) in 26 extended criteria human liver grafts undergoing hypothermic oxygenated perfusion to guide clinical graft assessment. Real-time data identified seven organs unsuitable for transplant that are discarded. The remaining grafts are transplanted and FMN values correlated with post-transplant i

SUBMITTER: Huwyler F 

PROVIDER: S-EPMC10427358 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature