Downregulation of YAP Activity Restricts P53 Hyperactivation to Promote Cell Survival in Confinement.

ABSTRACT: Cell migration through confining three dimensional (3D) topographies can lead to loss of nuclear envelope integrity, DNA damage, and genomic instability. Despite these detrimental phenomena, cells transiently exposed to confinement do not usually die. Whether this is also true for cells subjected to long-term confinement remains unclear at present. To investigate this, photopatterning and microfluidics are employed to fabricate a high-throughput device that circumvents limitations of previous cell confinement models and enables prolonged culture of single cells in microchannels with physiologically relevant length scales. The results of this study show that continuous exposure to tight confinement can trigger frequent nuclear envelope rupture events, which in turn promote P53 activation an

SUBMITTER: Hemmati F 

PROVIDER: S-EPMC10427377 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature