Reversible Parahydrogen Induced Hyperpolarization of ¹⁵ N in Unmodified Amino Acids Unraveled at High Magnetic Field.

ABSTRACT: Amino acids (AAs) and ammonia are metabolic markers essential for nitrogen metabolism and cell regulation in both plants and humans. NMR provides interesting opportunities to investigate these metabolic pathways, yet lacks sensitivity, especially in case of ¹⁵ N. In this study, spin order embedded in p-H₂ is used to produce on-demand reversible hyperpolarization in ¹⁵ N of pristine alanine and ammonia under ambient protic conditions directly in the NMR spectrometer. This is made possible by designing a mixed-ligand Ir-catalyst, selectively ligating the amino group of AA by exploiting ammonia as a strongly competitive co-ligand and preventing deactivation of Ir by bidentate ligation of AA. The stereoisomerism of the catalyst complexes is determined by hydrid

SUBMITTER: Vaneeckhaute E 

PROVIDER: S-EPMC10427394 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature