DNA storage in thermoresponsive microcapsules for repeated random multiplexed data access.

ABSTRACT: DNA has emerged as an attractive medium for archival data storage due to its durability and high information density. Scalable parallel random access to information is a desirable property of any storage system. For DNA-based storage systems, however, this still needs to be robustly established. Here we report on a thermoconfined polymerase chain reaction, which enables multiplexed, repeated random access to compartmentalized DNA files. The strategy is based on localizing biotin-functionalized oligonucleotides inside thermoresponsive, semipermeable microcapsules. At low temperatures, microcapsules are permeable to enzymes, primers and amplified products, whereas at high temperatures, membrane collapse prevents molecular crosstalk during amplification. Our data show that the platform outper

SUBMITTER: Bogels BWA 

PROVIDER: S-EPMC10427423 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature