Ontogenetically distinct neutrophils differ in function and transcriptional profile in zebrafish.

ABSTRACT: The current view of hematopoiesis considers leukocytes on a continuum with distinct developmental origins, and which exert non-overlapping functions. However, there is less known about the function and phenotype of ontogenetically distinct neutrophil populations. In this work, using a photoconvertible transgenic zebrafish line; Tg(mpx:Dendra2), we selectively label rostral blood island-derived and caudal hematopoietic tissue-derived neutrophils in vivo during steady state or upon injury. By comparing the migratory properties and single-cell expression profiles of both neutrophil populations at steady state we show that rostral neutrophils show higher csf3b expression and migration capacity than caudal neutrophils. Upon injury, both populations share a core transcriptional profile as well a

SUBMITTER: Garcia-Lopez JP 

PROVIDER: S-EPMC10427629 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature