Identification of circRNAs linked to Alzheimer's disease and related dementias.

ABSTRACT:

Introduction

Circular RNAs (circRNAs) exhibit selective expression in the brain and differential regulation in Alzheimer's disease (AD). To explore the role of circRNAs in AD, we investigated how circRNA expression varies between brain regions and with AD-related stress in human neuronal precursor cells (NPCs).

Methods

Ribosomal RNA-depleted hippocampus RNA-sequencing data were generated. Differentially regulated circRNAs in AD and related dementias were detected using CIRCexplorer3 and limma. circRNA results were validated using quantitative real-time PCR of cDNA from the brain and NPCs.

Results

We identified 48 circRNAs that were significantly associated with AD. We observed that circRNA expression differed by dementia subtype. Using NPCs, we demonstrated that expos

SUBMITTER: Puri S 

PROVIDER: S-EPMC10427739 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature