Central composite design driven optimization of sustainable stability indicating HPLC method for the determination of Tigecycline and greenness assessment.

ABSTRACT: Background: Tigecycline (TGC) is a recently developed antibiotic to battle resistant bacteria. The procedures outlined in the literature for analyzing TGC involve chemical solvents that could be hazardous. Therefore, this study aimed to create a sustainable and stable HPLC technique for quantifying Tigecycline in lyophilized powder. The powerful chemometric tool, experimental design (ED), will be applied to analyze the variables' interaction and impact on the selected analytical target profiles. Response surface methodology provides a tutorial on using the central composite design with three levels of variables and quadratic programming to optimize the design space of the developed method. Methods: The New HPLC method consisted of an aqueous buffer and ethanol as a green mobi

SUBMITTER: Hafez HM 

PROVIDER: S-EPMC10427804 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature