Pervasive genotype-by-environment interactions shape the fitness effects of antibiotic resistance mutations.

ABSTRACT: The fitness effects of antibiotic resistance mutations are a major driver of resistance evolution. While the nutrient environment affects bacterial fitness, experimental studies of resistance typically measure fitness of mutants in a single environment only. We explored how the nutrient environment affected the fitness effects of rifampicin-resistant rpoB mutations in Escherichia coli under several conditions critical for the emergence and spread of resistance-the presence of primary or secondary antibiotic, or the absence of any antibiotic. Pervasive genotype-by-environment (GxE) interactions determined fitness in all experimental conditions, with rank order of fitness in the presence and absence of antibiotics being strongly dependent on the nutrient environment. GxE intera

SUBMITTER: Soley JK 

PROVIDER: S-EPMC10427823 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature