Calcineurin B inhibits calcium oxalate crystallization, growth and aggregation via its high calcium-affinity property.

ABSTRACT: Calcineurin inhibitors (CNIs) are widely used in organ transplantation to suppress immunity and prevent allograft rejection. However, some transplant patients receiving CNIs have hypocitraturia, hyperoxaluria and kidney stone with unclear mechanism. We hypothesized that CNIs suppress activities of urinary calcineurin, which may serve as the stone inhibitor. This study aimed to investigate effects of calcineurin B (CNB) on calcium oxalate monohydrate (COM) stone formation. Sequence and structural analyses revealed that CNB contained four EF-hand (Ca²⁺-binding) domains, which are known to regulate Ca²⁺ homeostasis and likely to affect COM crystals. Various crystal assays revealed that CNB dramatically inhibited COM crystallization, crystal growth and crystal aggregation

SUBMITTER: Hadpech S 

PROVIDER: S-EPMC10427926 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature