Honokiol induces apoptosis-like death in Cryptocaryon irritans Tomont.

ABSTRACT:

Background

Cryptocaryon irritans, a common parasite in tropical and subtropical marine teleost fish, has caused serious harm to the marine aquaculture industry. Honokiol was proven to induce C. irritans tomont cytoplasm shrinkage and death in our previous study, but the mechanism by which it works remains unknown.

Methods

In this study, the changes of apoptotic morphology and apoptotic ratio were detected by microscopic observation and AnnexinV-FITC/PI staining. The effects of honokiol on intracellular calcium ([Ca²⁺]_i) concentration, mitochondrial membrane potential (ΔΨm), reactive oxygen species (ROS), quantity of DNA fragmentations (QDF) and caspase activities were detected by Fluo-3 staining, JC-1 staining, DCFH-DA staining, Tunel method and caspase

SUBMITTER: Zhao ZC 

PROVIDER: S-EPMC10428556 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature