M2 exosomes modified by hydrogen sulfide promoted bone regeneration by moesin mediated endocytosis.

ABSTRACT: Bone defects caused by trauma or tumor led to high medical costs and poor life quality for patients. The exosomes, micro vesicles of 30-150 nm in diameter, derived from macrophages manipulated bone regeneration. However, the role of hydrogen sulfide (H₂S) in the biogenesis and function of exosomes and its effects on bone regeneration remains elusive. In this study, we used H₂S slow releasing donor GYY4137 to stimulate macrophages and found that H₂S promoted the polarization of M2 macrophages to increase bone regeneration of MSCs in vitro and in vivo. Moreover, we developed the H₂S pre-treated M2 macrophage exosomes and found these exosomes displayed significantly higher capacity to promote bone regeneration in calvarial bone defects

SUBMITTER: Zhou YK 

PROVIDER: S-EPMC10429289 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature