Effects of sevoflurane and its metabolite hexafluoroisopropanol on hypoxia/reoxygenation-induced injury and mitochondrial bioenergetics in murine cardiomyocytes.

ABSTRACT:

Background

The volatile anaesthetic sevoflurane protects cardiac tissue from reoxygenation/reperfusion. Mitochondria play an essential role in conditioning. We aimed to investigate how sevoflurane and its primary metabolite hexafluoroisopropanol (HFIP) affect necrosis, apoptosis, and reactive oxygen species formation in cardiomyocytes upon hypoxia/reoxygenation injury. Moreover, we aimed to describe the similarities in the mode of action in a mitochondrial bioenergetics analysis.

Methods

Murine cardiomyocytes were exposed to hypoxia (0.2% O₂ for 6 h), followed by reoxygenation (air with 5% CO₂ for 2 h) in the presence or absence sevoflurane 2.2% or HFIP 4 mM. Lactate dehydrogenase (LDH) release (necrosis), caspase activation (apoptosis), reactive oxygen

SUBMITTER: Roth Z'graggen B 

PROVIDER: S-EPMC10430838 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature