Experimental Study of Warburg Effect in Keloid Nodules: Implication for Downregulation of miR-133b.

ABSTRACT:

Background

A keloid is composed of several nodules, which are divided into two zones: the central zone (CZ; a hypoxic region) and the marginal zone (MZ; a normoxic region). Keloid nodules play a key role in energy metabolic activity for continuous growth by increasing in number and total area. In this study, we aimed to investigate the roles of the zones in the execution of the Warburg effect and identify which microRNAs regulate this phenomenon in keloid tissue.

Methods

Eleven keloids from patients were used. Using immunohistochemical analysis, 179 nodules were randomly chosen from these keloids to identify glycolytic enzymes, autophagic markers, pyruvate kinase M (PKM) 1/2, and polypyrimidine tract binding protein 1 (PTBP1). Western blot and qRT-PCR tests were also perform

SUBMITTER: Lee Y 

PROVIDER: S-EPMC10431320 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature