Profiling single cancer cell metabolism via high-content SRS imaging with chemical sparsity.

ABSTRACT: Metabolic reprogramming in a subpopulation of cancer cells is a hallmark of tumor chemoresistance. However, single-cell metabolic profiling is difficult because of the lack of a method that can simultaneously detect multiple metabolites at the single-cell level. In this study, through hyperspectral stimulated Raman scattering (hSRS) imaging in the carbon-hydrogen (C-H) window and sparsity-driven hyperspectral image decomposition, we demonstrate a high-content hSRS (h²SRS) imaging approach that enables the simultaneous mapping of five major biomolecules, including proteins, carbohydrates, fatty acids, cholesterol, and nucleic acids at the single-cell level. h²SRS imaging of brain and pancreatic cancer cells under chemotherapy revealed acute and adapted chemotherapy-ind

SUBMITTER: Tan Y 

PROVIDER: S-EPMC10431717 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature