Fanzor is a eukaryotic programmable RNA-guided endonuclease.

ABSTRACT: RNA-guided systems, which use complementarity between a guide RNA and target nucleic acid sequences for recognition of genetic elements, have a central role in biological processes in both prokaryotes and eukaryotes. For example, the prokaryotic CRISPR-Cas systems provide adaptive immunity for bacteria and archaea against foreign genetic elements. Cas effectors such as Cas9 and Cas12 perform guide-RNA-dependent DNA cleavage¹. Although a few eukaryotic RNA-guided systems have been studied, including RNA interference² and ribosomal RNA modification³, it remains unclear whether eukaryotes have RNA-guided endonucleases. Recently, a new class of prokaryotic RNA-guided systems (termed OMEGA) was reported^4,5. The OMEGA effector TnpB is the putative ance

SUBMITTER: Saito M 

PROVIDER: S-EPMC10432273 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature