Zwitterionic microgel preservation platform for circulating tumor cells in whole blood specimen.

ABSTRACT: The immediate processing of whole blood specimen is required in circulating tumor cell-based liquid biopsy. Reliable blood specimen stabilization towards preserving circulating tumor cells can enable more extensive geographic sharing for precise rare-cell technology, but remains challenging due to the fragility and rarity of circulating tumor cells. Herein, we establish a zwitterionic magnetic microgel platform to stabilize whole blood specimen for long-term hypothermic preservation of model circulating tumor cells. We show in a cohort study of 20 cancer patients that blood samples can be preserved for up to 7 days without compromising circulating tumor cell viability and RNA integrity, thereby doubling the viable preservation duration. We demonstrate that the 7-day microgel-preserved bloo

SUBMITTER: Ma Y 

PROVIDER: S-EPMC10432405 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature