Micro- and nanochamber array system for single enzyme assays.

ABSTRACT: Arrays of small reaction containers, ranging from 624 femtoliters (10^-15 L) to 270 attoliters (10^-18 L), for capturing a single enzyme molecule and measuring the activity were developed along with a new reversible sealing system based on a pneumatic valve actuator made of polydimethylsiloxane (PDMS). The valve was actuated by PBS solution, effectively preventing evaporation of the solution from the micro- and nanochambers and allowing the assay to be performed over a long period of time. The hydrolysis rates of β-D-galactosidase (β-gal), k_cat, were decreased according to the decrease of the chamber size, and the overall tendency seems to be symmetrically related to the specific surface area of the chambers even under the prevented condition of non-specific

SUBMITTER: Iijima K 

PROVIDER: S-EPMC10432523 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature