Particle fusion of super-resolution data reveals the unit structure of Nup96 in Nuclear Pore Complex.

ABSTRACT: Single molecule localization microscopy offers resolution nearly down to the molecular level with specific molecular labelling, and is thereby a promising tool for structural biology. In practice, however, the actual value to this field is limited primarily by incomplete fluorescent labelling of the structure. This missing information can be completed by merging information from many structurally identical particles in a particle fusion approach similar to cryo-EM single-particle analysis. In this paper, we present a data analysis of particle fusion results of fluorescently labelled Nup96 nucleoporins in the Nuclear Pore Complex to show that Nup96 occurs in a spatial arrangement of two rings of 8 units with two Nup96 copies per unit giving a total of 32 Nup96 copies per pore. We use Artifi

SUBMITTER: Wang W 

PROVIDER: S-EPMC10432550 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature