Trastuzumab-induced human cardiomyocyte damage through the Notch2/JAK2/STAT3 pathway.

ABSTRACT:

Objective

Trastuzumab is the preferred drug for the treatment of breast cancer. However, research on the cellular mechanisms of trastuzumab's potential cardiotoxicity is insufficient. The purpose of this study was to explore the toxic effects and potential mechanism of action of trastuzumab on cardiomyocytes.

Method

Human Cardiomyocyte (HCM) viability was assessed using the MTT method. HCM apoptosis was detected using the Hoechst33342/PI Fluorescent staining. The LDH and CK activities of the cell were measured using commercially available LDH and CK assay kits. The expression levels of Notch2, JAK2, STAT3, cleaved caspase 3, bax, and bcl 2 in HCMs were detected using western blotting.

Results

The results showed that 250 mg/L trastuzumab induced cardiomyocyte injury an

SUBMITTER: Su Z 

PROVIDER: S-EPMC10432602 | biostudies-literature | 2023 Jan-Dec

REPOSITORIES: biostudies-literature