EV-mediated promotion of myogenic differentiation is dependent on dose, collection medium, and isolation method.

ABSTRACT: Extracellular vesicles (EVs) have been implicated in the regulation of myogenic differentiation. C2C12 murine myoblast differentiation was reduced following treatment with GW4869 or heparin (to inhibit exosome biogenesis and EV uptake, respectively). Conversely, treatment with C2C12 myotube-conditioned medium enhanced myogenic differentiation. Ultrafiltration-size exclusion liquid chromatography (UF-SEC) was used to isolate EVs and non-EV extracellular protein in parallel from C2C12 myoblast- and myotube-conditioned medium. UF-SEC-purified EVs promoted myogenic differentiation at low doses (≤2 × 10⁸ particles/mL) and were inhibitory at the highest dose tested (2 × 10¹¹ particles/mL). Conversely, extracellular protein fractions had no effect on myogenic differentiation

SUBMITTER: Hanson B 

PROVIDER: S-EPMC10432918 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature