The large extracellular loop is important for recruiting CD63 to exosomes.
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ABSTRACT:
SUBMITTER: Ivanusic D
PROVIDER: S-EPMC10432940 | biostudies-literature | 2023
REPOSITORIES: biostudies-literature
Publications
The large extracellular loop is important for recruiting CD63 to exosomes.
Ivanusic Daniel D Denner Joachim J
microPublication biology 20230803
Exosomes are small extracellular vesicles that are secreted from cells. To characterize exosome fraction marker proteins of the tetraspanin family in particular, CD9, CD63, and CD81 are routinely used. CD63 expression constructs were employed to investigate the influence of the large extracellular loop (LEL) of CD63 on sorting into exosomes. When the LEL of CD63 fused with mCherry was deleted, the protein was no longer found in the purified exosome fraction. This finding demonstrates the importa ...[more]
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