An open-source smart fraction collector for isocratic preparative liquid chromatography

ABSTRACT: Graphical abstract Preparative liquid chromatography is a technique for separating complex samples or isolating pure compounds from complex extracts. It involves eluting samples through a packed column and selectively collecting or isolating the separated bands in a sequence of fractions. Depending on the column length and the sample complexity, a large number of fractions may be obtained, making fraction collection a laborious and time-consuming process. Manual fraction collection is also tedious, error-prone, less reproducible, and susceptible to contamination. Several commercial and lab-made solutions are available for automated fraction collection, but most systems do not synchronize with the instrument detector and collect fractions at fixed volumes or time intervals. We have assembl

SUBMITTER:  

PROVIDER: S-EPMC10432948 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature