SARS-CoV-2 specific plasma cells acquire long-lived phenotypes in human bone marrow.

ABSTRACT:

Background

SARS-CoV-2 specific antibody-secreting plasma cells (PC) mediating specific humoral immunity have been identified in the human bone marrow (BM) after COVID-19 or vaccination against SARS-CoV-2. However, it remained unclear whether or not they acquire phenotypes of human memory plasma cells.

Methods

SARS-CoV-2-specific human bone marrow plasma cells (BMPC) were characterised by tetramer-based, antigen-specific flow cytometry and FluoroSpot assay.

Findings

SARS-CoV-2 spike-S1-specific PC were detectable in all tested BM samples of previously vaccinated individuals, representing 0.22% of total BMPC. The majority of SARS-CoV-2-specific BMPC expressed IgG and their specificity for the spike S1 protein indicated emergence from a systemic vaccination response. Of

SUBMITTER: Schulz AR 

PROVIDER: S-EPMC10432952 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature