Molecular epidemiology and genomic characterization of a plasmid-mediated mcr-10 and bla_NDM-1 co-harboring multidrug-resistant Enterobacter asburiae.

ABSTRACT: Colistin is considered as one of the last-resort antimicrobial agents for treating multidrug-resistant bacterial infections. Multidrug-resistant E. asburiae has been increasingly isolated from clinical patients, which posed a great challenge for antibacterial treatment. This study aimed to report a mcr-10 and bla_NDM-1 co-carrying E. asburiae clinical isolate 5549 conferred a high-level resistance against colistin. Antibiotic susceptibility testing was performed using the microdilution broth method. Transferability of mcr-10 and bla_NDM-1-carrying plasmids were investigated by conjugation experiments. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was used to identify modifications in

SUBMITTER: Li X 

PROVIDER: S-EPMC10433016 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature