Evidence of a role for CutRS and actinorhodin in the secretion stress response in Streptomyces coelicolor M145.

ABSTRACT: CutRS was the first two-component system to be identified in Streptomyces species and is highly conserved in this genus. It was reported >25 years ago that deletion of cutRS increases the production of the antibiotic actinorhodin in Streptomyces coelicolor. However, despite this early work, the function of CutRS has remained enigmatic until now. Here we show that deletion of cutRS upregulates the production of the actinorhodin biosynthetic enzymes up to 300-fold, explaining the increase in actinorhodin production. However, while ChIP-seq identified 85 CutR binding sites in S. coelicolor none of these are in the actinorhodin biosynthetic gene cluster, meaning the effect is indirect. The directly regulated CutR targets identified in this study are implicate

SUBMITTER: McLean TC 

PROVIDER: S-EPMC10433416 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature