The distribution of fitness effects of plasmid pOXA-48 in clinical enterobacteria.

ABSTRACT: Antimicrobial resistance (AMR) in bacteria is a major public health problem. The main route for AMR acquisition in clinically important bacteria is the horizontal transfer of plasmids carrying resistance genes. AMR plasmids allow bacteria to survive antibiotics, but they also entail physiological alterations in the host cell. Multiple studies over the last few years have indicated that these alterations can translate into a fitness cost when antibiotics are absent. However, due to technical limitations, most of these studies are based on analysing new associations between plasmids and bacteria generated in vitro, and we know very little about the effects of plasmids in their native bacterial hosts. In this study, we used a CRISPR-Cas9-tool to selectively cure plasmids from clinical

SUBMITTER: Fernandez-Calvet A 

PROVIDER: S-EPMC10433420 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature