RNA N6‑methyladenosine methyltransferase WTAP promotes the differentiation of endothelial progenitor cells.

ABSTRACT: N6-methyladenosine (m⁶A) serves a critical role in regulating gene expression and has been associated with various diseases; however, its role in the differentiation of endothelial progenitor cells (EPCs) remains unclear. The present study used liquid chromatography with tandem mass spectrometry and immunofluorescence assays to quantify the levels of m⁶A in human peripheral blood-derived EPCs (HPB-EPCs) before and after differentiation into mature cells. The present study performed Cell Counting Kit 8, Transwell, and tube formation assays to determine the effects of overexpression and knockdown of Wilms' tumor 1-associated protein (WTAP) on HPB-EPCs. The results revealed that the level of m⁶A modification was significantly increased during HPB-EPCs differen

SUBMITTER: Wu L 

PROVIDER: S-EPMC10433437 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature