CCNB1 is a novel prognostic biomarker and promotes proliferation, migration and invasion in Wilms tumor.

ABSTRACT:

Background

Wilms tumour (WT) is a mixed type of embryonal tumour that usually occurs in early childhood. However, our knowledge of the pathogenesis or progression mechanism of WT is inadequate, and there is a scarcity of beneficial therapeutic strategies.

Methods

High-throughput RNA sequencing was employed in this study to identify differentially expressed genes (DEGs) in clinical tumor samples and matching normal tissues. The STRING database was utilized to build a protein-protein interaction (PPI) network, and the Cytohubba method was used to identify the top 10 highly related HUB genes. Then, the key genes were further screened by univariate COX survival analysis. Subsequently, the XCELL algorithm was used to evaluate the tumour immune infiltration. RT-PCR, WB, and IF wer

SUBMITTER: Xiang B 

PROVIDER: S-EPMC10433552 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature