Dissecting the molecular trajectory of fibroblast reprogramming to chemically induced mammary epithelial cells.

ABSTRACT: Introduction: The plasticity of cell identity allows cellular reprogramming that manipulates the lineage of cells to generate the target cell types, bringing new avenues for disease modeling and autologous tailored cell therapy. Previously, we had already successfully established a technical platform for inducing fibroblast reprogramming to chemically induced mammary epithelial cells (CiMECs) by small-molecule compounds. However, exactly how the molecular mechanism driving the lineage conversion remains unknown. Methods: We employ the RNA-sequencing technology to investigate the transcriptome event during the reprogramming process and reveal the molecular mechanisms for the fate acquisition of mammary lineage. Results: The multi-step reprogramming process first overcom

SUBMITTER: Qin L 

PROVIDER: S-EPMC10433763 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature