Implication of the σ^E Regulon Members OmpO and σ^N in the ΔompA_299-356-Mediated Decrease of Oxidative Stress Tolerance in Stenotrophomonas maltophilia.

ABSTRACT: Outer membrane protein A (OmpA) is the most abundant porin in bacterial outer membranes. KJΔOmpA_299-356, an ompA C-terminal in-frame deletion mutant of Stenotrophomonas maltophilia KJ, exhibits pleiotropic defects, including decreased tolerance to menadione (MD)-mediated oxidative stress. Here, we elucidated the underlying mechanism of the decreased MD tolerance mediated by ΔompA_299-356. The transcriptomes of wild-type S. maltophilia and the KJΔOmpA_299-356 mutant strain were compared, focusing on 27 genes known to be associated with oxidative stress alleviation; however, no significant differences were identified. OmpO was the most downregulated gene in KJΔOmpA_299-356. KJΔOmpA_299-356 complementation with the chromo

SUBMITTER: Ku RH 

PROVIDER: S-EPMC10433810 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature