Short- and Long-Read Sequencing Reveals the Presence and Evolution of an IncF Plasmid Harboring bla_CTX-M-15 and bla_CTX-M-27 Genes in Escherichia coli ST131.

ABSTRACT: Escherichia coli sequence type 131 (ST131) has contributed to the spread of extended-spectrum beta-lactamase (ESBL) and has emerged as the dominant cause of hospital- and community-acquired urinary tract infections. Here, we report for the first time an in-depth analysis of whole-genome sequencing (WGS) of 4 ESBL-producing E. coli ST131 isolates recovered from patients in two hospitals in Armenia using Illumina short-read sequencing for accurate base calling to determine their genotype and to infer their phylogeny and using Oxford Nanopore Technologies long-read sequencing to resolve plasmid and chromosomal genetic elements. Genotypically, the four Armenian isolates were identified as part of the H30Rx/clade C2 (n = 2) and H41/clade A (n = 2) lineages and were p

SUBMITTER: Cave R 

PROVIDER: S-EPMC10433869 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature