Identification and Molecular Characteristics of a Novel Single-Stranded RNA Virus Isolated from Culex tritaeniorhynchus in China.

ABSTRACT: Hubei mosquito virus 2 (HMV2) is a novel mosquito virus that was first identified in 2016 in Hubei Province, China. Until now, HMV2 has been shown to be endemic in some areas of China and Japan, but its biological characteristics, epidemiology, and pathogenicity are not yet known. This report describes the detection of HMV2 in mosquitoes that were collected in Shandong Province in 2019 and presents the first isolation and molecular characterization of the virus. In this study, a total of 2,813 mosquitoes were collected and then divided into 57 pools, according to location and species. qRT-PCR and nested PCR were performed to confirm the presence of HMV2, and its genomic features, phylogenetic relationships, growth characteristics, and potential pathogenicity were further analyzed. The resu

SUBMITTER: Wu Z 

PROVIDER: S-EPMC10433992 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature