CRISPR-Cas13a-Based Assay for Accurate Detection of OXA-48 and GES Carbapenemases.

ABSTRACT: Carbapenem-resistant pathogens have been recognized as a health concern as they are both difficult to treat and detect in clinical microbiology laboratories. Researchers are making great efforts to develop highly specific, sensitive, accurate, and rapid diagnostic techniques, required to prevent the spread of these microorganisms and improve the prognosis of patients. In this context, CRISPR-Cas systems are proposed as promising tools for the development of diagnostic methods due to their high specificity; the Cas13a endonuclease can discriminate single nucleotide changes and displays collateral cleavage activity against single-stranded RNA molecules when activated. This technology is usually combined with isothermal pre-amplification reactions in order to increase its sensitivity. We have

SUBMITTER: Ortiz-Cartagena C 

PROVIDER: S-EPMC10434040 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature