Soybean and Cotton Spermosphere Soil Microbiome Shows Dominance of Soilborne Copiotrophs.

ABSTRACT: The spermosphere is the transient, immediate zone of soil around imbibing and germinating seeds. It represents a habitat where there is contact between seed-associated microbes and soil microbes, but it is studied less than other plant habitats. Previous studies on spermosphere microbiology were primarily culture based or did not sample the spermosphere soil as initially defined in space and time. Thus, the objectives of this study were to develop an efficient strategy to collect spermosphere soils around imbibing soybean and cotton in nonsterile soil and investigate changes in microbial communities. The method employed sufficiently collected spermosphere soil as initially defined in space by constraining the soil sampled with a cork borer and confining the soil to a 12-well microtiter pla

SUBMITTER: Olofintila OE 

PROVIDER: S-EPMC10434258 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature