MicroRNA-203 inhibits migration and invasion of canine tonsillar squamous cell carcinoma cells by targeting SLUG.

ABSTRACT:

Objective

Squamous cell carcinoma (SCC) occurring in the tonsils (TSCC) has a poorer prognosis than SCC occurring in other regions of the oral cavity (non-tonsillar SCC [NTSCC]) because it easily metastasizes to distant organs. This study aimed to elucidate the molecular mechanisms underlying the migration and invasion of TSCC cells in vitro.

Materials and methods

This study focused on differential microRNA (miRNA) expression using microRNA microarrays and quantitative polymerase chain reaction in canine TSCC and NTSCC tissues and cell lines. A target gene of the miRNA involved in cell migration and invasion was validated by wound healing, transwell, and luciferase assays.

Results

miR-203 expression was lower in TSCC tissues than in the normal oral mucosa and N

SUBMITTER: Noguchi S 

PROVIDER: S-EPMC10434855 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature