Gut-derived peptide hormone receptor expression in the developing mouse hypothalamus.

ABSTRACT:

Objective

In adult organisms, a number of receptors have been identified which modulate metabolic processes related to peptides derived from the intestinal tract. These receptors play significant roles in glucose homeostasis, food intake and energy balance. Here we assess these classical metabolic receptors and their expression as well as their potential role in early development of hypothalamic neuronal circuits.

Methods

Chow-fed C57BL6/N female mice were mated and hypothalamic tissue was collected from offspring across postnatal development (postnatal day 7-21). Subsequent qPCR and Western Blot analyses were used to determine mRNA and protein changes in gut-derived peptide hormone receptors. Correlations to body weight, blood glucose and circulating leptin levels were anal

SUBMITTER: Cantacorps L 

PROVIDER: S-EPMC10434938 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature