Morphological phenotyping after mouse whole embryo culture

ABSTRACT: Morphological phenotyping of the mouse embryo is described at neurulation stages, primarily as a guide to evaluating the outcome of whole embryo cultures between embryonic days 8.5 and 9.5. During this period, neural tube closure is initiated and progresses to completion in the cranial region. Spinal closure is still underway at the end of the culture period. The focus of this article is particularly on phenotyping that can be performed at the bench, using a stereomicroscope. This involves assessment of embryonic health, through observation and scoring of yolk sac blood circulation, measurement of developmental stage by somite counting, and determination of crown-rump length as a measure of growth. Axial rotation (“turning”) can also be assessed using a simple scoring system. Neural tube c

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PROVIDER: S-EPMC10435082 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature