Establishment of a human microbiome- and immune system-reconstituted dual-humanized mouse model.

ABSTRACT: Humanized mice are widely used to study the human immune system in vivo and investigate therapeutic targets for various human diseases. Immunodeficient NOD/Shi-scid-IL2rγ^null (NOG) mice transferred with human hematopoietic stem cells are a useful model for studying human immune systems and analyzing engrafted human immune cells. The gut microbiota plays a significant role in the development and function of immune cells and the maintenance of immune homeostasis; however, there is currently no available animal model that has been reconstituted with human gut microbiota and immune systems in vivo. In this study, we established a new model of CD34⁺ cell-transferred humanized germ-free NOG mice using an aseptic method. Flow cytometric analysis revealed that the germ-free h

SUBMITTER: Ka Y 

PROVIDER: S-EPMC10435363 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature