Inactivation kinetics of horseradish peroxidase (HRP) by hydrogen peroxide.

ABSTRACT: In recent years, the peroxidase enzymes have generated wide interest in several industrial processes, such as wastewater treatments, food processing, pharmaceuticals, and the production of fine chemicals. However, the low stability of the peroxidases in the presence of hydrogen peroxide (H₂O₂) has limited its commercial use. In the present work, the effect of H₂O₂ on the inactivation of horseradish peroxidase (HRP) was evaluated. Three states of HRP (E₀, E₂, and E₃) were identified. While in the absence of H₂O₂, the resting state E₀ was observed, in the presence of low and high concentrations of H₂O₂, E_2, and E₃ were found, respectively.

SUBMITTER: Morales-Urrea D 

PROVIDER: S-EPMC10435507 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature