Transcriptional profiling of canine osteosarcoma identifies prognostic gene expression signatures with translational value for humans.

ABSTRACT: Canine osteosarcoma is increasingly recognized as an informative model for human osteosarcoma. Here we show in one of the largest clinically annotated canine osteosarcoma transcriptional datasets that two previously reported, as well as de novo gene signatures devised through single sample Gene Set Enrichment Analysis (ssGSEA), have prognostic utility in both human and canine patients. Shared molecular pathway alterations are seen in immune cell signaling and activation including TH1 and TH2 signaling, interferon signaling, and inflammatory responses. Virtual cell sorting to estimate immune cell populations within canine and human tumors showed similar trends, predominantly for macrophages and CD8+ T cells. Immunohistochemical staining verified the increased presence of immune cells in tum

SUBMITTER: Mannheimer JD 

PROVIDER: S-EPMC10435536 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature