Aptamer-based assay for rapid detection, surveillance, and screening of pathogenic Leptospira in water samples.

ABSTRACT: Leptospirosis is a potentially fatal waterborne infection caused by Leptospira interrogans, impacting both humans and animals in tropical regions. However, current diagnostic methods for detecting pathogenic Leptospira have sensitivity, cost, and time limitations. Therefore, there is a critical need for a rapid, sensitive, and cost-effective detection method. This study presents the development of an aptamer-based assay for pathogenic Leptospira detection. Aptamers targeting Leptospira were generated using the SELEX method and screened for binding affinity with major Leptospiral outer membrane proteins through in silico analysis. The aptamer with the highest binding affinity was selected for further evaluation. To enable visual detection, the aptamer was conjugated to gold nanoparticles (A

SUBMITTER: Vishwakarma A 

PROVIDER: S-EPMC10435560 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature