Fertility restoration in mice with chemotherapy induced ovarian failure using differentiated iPSCs.

ABSTRACT:

Background

Treatment options for premature ovarian insufficiency (POI) are limited to hormone replacement and donor oocytes. A novel induced pluripotent stem cell (iPSC) transplant paradigm in a mouse model has potential translational applications for management of POI.

Methods

Mouse ovarian granulosa cell derived-iPSCS were labelled with green fluorescent protein (GFP) reporter and differentiated in vitro into oocytes. Differentiated cells were assayed for estradiol and progesterone secretion by enzyme-linked immunosorbent assays. After Fluorescence-Activated Cell Sorting (FACS) for the cell surface marker anti-Mullerian hormone receptor (AMHR2), enriched populations of differentiated cells were surgically transplanted into ovaries of mice that had POI secondary to gonadoto

SUBMITTER: Elias KM 

PROVIDER: S-EPMC10435842 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature